|
Phosphorylated residues on Cdc25 and Wee1. |
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| Xenopus Site |
Human Site |
+/- |
Kinase |
Proposed function |
References |
|
|
|
||||||
| Cdc25 |
T48 |
+ |
Cdc2, ERK |
Enhances activity and required for Cdc2 activation, binds to Pin1 |
22, 29, 48, 50, 51 |
|
| T67 |
+ |
Cdc2, ? |
Required for Cdc2 activation, binds to Pin1 |
22, 48, 50, 51 |
||
| T138 |
T130 |
+ |
Cdk2, ERK |
Promotes 14-3-3 release, may create binding site for Plk1 |
21, 29, 31, 32 |
|
| S198 |
+ |
Plk1 |
Promotes nuclear translocation |
41 |
||
| S205 |
S168 |
+/- |
ERK, p38/JNK |
Increases activity in Xenopus/Inhibits activity in mammalian cells |
29, 54, 53 |
|
| S285 |
S214 |
+ |
Cdc2 |
Promotes PP1-mediated dephosphorylation of S287 |
46–48 |
|
| S287 |
S216 |
- |
Chk1, Chk2, C-TAK, CamKII, PKA |
Binding site for 14-3-3, blocks nuclear import |
5–20, 58 |
|
|
|
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| Wee1 |
S38 |
- |
Cdc2 |
Promotes degradation via Tome-1 |
83, 86 |
|
| T53 |
- |
Cdc2 |
Regulates protein stability |
83 |
||
| S53 |
- |
Plk1 |
Promotes degradation via β-TrCP |
87, 88 |
||
| Y90 |
+ |
Wee1 |
Promotes activity |
78 |
||
| Y103 |
+ |
Wee1 |
Promotes activity |
78 |
||
| T104 |
- |
Cdc2 |
Required for Wee1 inactivation |
83 |
||
| Y110 |
+ |
Wee1 |
Promotes activity |
78 |
||
| S121 |
- |
CK2 |
Promotes degradation via β-TrCP |
87 |
||
| S123 |
- |
Cdc2 |
Promotes Plk1 binding and degradation via β-TrCP |
87, 88 |
||
| T150 |
- |
Cdc2, ? |
Required for Wee1 inactivation, binds top Pin1 |
83, 84 |
||
| Y295 |
+ |
Wee1 |
Promotes activity |
77 |
||
| Y362 |
+ |
Wee1 |
Promotes activity |
77 |
||
| S549 |
S642 |
+/- |
Chk1/Akt |
Promotes 14-3-3 binding and increases activity/Promotes cytoplasmic localization |
58, 75–77 |
|
|
The effect of phosphorylation at specific residues is listed as activating (+) or inactivating (-). | ||||||
Perry and Kornbluth Cell Division 2007 2:12 doi:10.1186/1747-1028-2-12 |
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