Genetic analysis of the spindle checkpoint genes san-1, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans

Vinita A Hajeri¹ , Anil M Stewart¹ , Landon L Moore² and Pamela A Padilla¹

¹Department of Biological Sciences, University of North Texas, Denton, TX, USA

²Department of Genetics and Genomics, Boston University School of Medicine, Boston, MA, USA

author email corresponding author email

Cell Division 2008, 3:6doi:10.1186/1747-1028-3-6


Published:	4 February 2008

Additional files

Additional file 1:

The Y54G9A.6 gene encodes the putative BUB-3 protein. A multiple sequence alignment using ClustalW indicates that the Y54G9A.6 gene in C. elegans encodes a protein (BUB-3) with homology to the spindle checkpoint protein BUB3. The amino acid identity between the C. elegans putative BUB-3 protein and other BUB3 proteins is 44% for H. sapiens, 43% for D. melanogastor and 19% for S. cerevisiae. Identical amino acids have a black background shade.

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Additional file 2:

Live cell imaging of san-1(ok1580);hcp-1(RNAi);tbg-1::GFP;pie-1::GFP::H2B embryos. A spinning disc confocal microscope to analyze chromosome segregation in the san-1(ok1580);hcp-1(RNAi) animals. Images were collected, processed using NIH Image and imported into Quick Time for display. In this embryo there are two blastomeres that progress through metaphase. One of the blastomeres displays normal mitotic progression (top metaphase blastomere) whereas another blastomere displays abnormal chromosome segregation (bottom metaphase blastomere) leading to anaphase bridging.

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