Figure 3.

Spatial and temporal organization of protein-modifying enzymes at the bud neck of Saccharomyces cerevisiae. Gene products known or predicted to have the capacity to modify other proteins and that have been visualized at the bud neck by fluorescence microscopy are listed along with the stages of the mitotic division cycle at which they are found at the neck (orange bars), and the region of the bud neck to which they localize (green), where known. Also indicated are the time when emergence of the bud first becomes visible (dashed lined) and the time period corresponding to disassembly of the mitotic spindle and completion of the septum (grey bar). Smt3 is the yeast ortholog of SUMO. It should be noted that this list does not include certain enzymes known to act on septins with important functional consequences (e.g, Cla4 [10]) that do not stably associate with the bud neck, and instead localize to, but quickly depart from, the future site of bud emergence [78]. See Table 1 for citations of the appropriate supporting literature. Adapted from [60] with permission from Elsevier.