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Table 1
*Protein-modifying enzymes and their targets at the Saccharomyces cerevisiae* bud neck**
Gene product	Closest mammalian ortholog	Function	Neck substrate(s)	Reference(s)

Protein kinases
Cbk1	NDR2	NDR family; cell wall morphogenesis, cell separation	Ace2	[79]
Cdc5	PLK2	Polo/PLK family; cell cycle regulation, cytokinesis, mitotic exit	Swe1	[80]
Cdc15	MST1	STE20-like family; activated by GTP-bound Tem1, late nuclear division, mitotic exit	Dbf2	[81]
Cdc28	CDK1	Catalytic subunit of Cdk1; cell cycle progression	Cdc3 Shs1 Swe1	[48,50,82]
Clb2	Cyclin B2	B-type cyclin; regulatory subunit for Cdc28; cell cycle progression	---	[83]
Dbf2 Dbf20	LATS1	NDR family; anaphase-telophase, mitotic exit	Cdc14	[84]
Elm1	LKB1	Upstream activator of Snf1 and other AMPK-like protein kinases involved in septin assembly	Gin4 Hsl1 Kcc4	[85,86]
Gin4	BRSK1	Nim1-related and AMPK-like family; septin collar assembly	Shs1 Swe1?	[49]
Hrr25	CKIδ	Casein kinase I family; located throughout the cell, but concentrated at bud tip and bud neck	?	[87]
Hsl1	BRSK2	Nim1-related and AMPK-like family; morphogenesis checkpoint, Swe1 degradation	Shs1 Swe1	Trott AE, Gullbrand B & Thorner J, unpublished results
Kcc4	BRSK1	Nim1-related and AMPK-like family; septin collar assembly	Swe1?	[88]
Kin4	MARK4	AMPK-like family; negatively regulates mitotic exit in response to a misaligned spindle	Gic2? Hsl1?	[89,90]
Mob1	MATS1	Regulatory and substrate targeting subunit of Dbf2 (and Dbf20)	---	[91]
Mob2	MOB1A	Regulatory and substrate targeting subunit of Cbk1	---	[91]
Pcl1 Pcl2 Pcl9	Cyclin A/B	AB-type cyclins; regulatory subunits for Pho85; cell morphogenesis	---	[92-94]
Pho85	CDK5	Catalytic subunit; transcription, cell morphogenesis	Bni4 Cdc3 Cdc10 Cdc12	[33,95]
Pkc1	PRK2	Cell wall integrity; activated by GTP-bound Rho1, localizes to bud neck late in the cell cycle before cell separation	?	[96]
Rad53	CHK2	DNA damage checkpoint	Shs1 Cdc12	[97]
Swe1	WEE1	Morphogenesis checkpoint	Cdc28	[98]
Yck2	CKIγ	Casein kinase I family; a peripheral plasma membrane protein anchored by C-terminal palmitoylation	?	[99]
Phosphatases
Afr1	PHACTR4	Regulatory and substrate targeting subunit of phosphoprotein phosphatase (PP) 1; septin reorganization during mating projection formation	---	[100]
Cdc55	B55β2	B-type regulatory and substrate targeting subunit of PP2A	---	[101]
Glc7	PP1	Catalytic subunit of PP1	?	[102]
Pph21 Pph22	PP2ACα PP2ACβ	Catalytic subunit isoforms of PP2A	?	[101,103]
Rts1	B56δ1	B'-type regulatory and substrate targeting subunit of PP2A	Shs1	[53]
Tpd3	PR65α	A-type regulatory (scaffold) subunit of PP2A	---	[103]
Other modifiers
Grr1	FBXL20	F-box protein for substrate recognition by an SCF (Skp1—Cdc53—Cdc34)-type ubiquitin-protein ligase (E3), targets landmark proteins for destruction after septin ring site is established	Gic1? Gic2	[104,105]
Hsl7	JBP1	PRMT5; type II protein-arginine methyltransferase that generates symmetric N, N'-dimethylarginine residues in its substrates	Swe1?	[106,107]
Siz1 Siz2/Nfi1	PIAS2 PIAS4	RING-like domain-containing SUMO-protein ligases (E3); responsible for mother side-specific attachment of SUMO to septins in the collar at the neck at the onset of anaphase	Cdc3 Cdc11 Shs1	[61,76]
Mms21	-none-	U box domain-containing SUMO-protein ligase (E3); capable of attaching SUMO to septins when Siz1 & Siz2 absent	Cdc3 Cdc11 Shs1	[63]
Smt3	SUMO-1	Small ubiquitin-like modifier; may contribute to regulating disassembly of septin collar and/or association of other proteins with septins in the collar	---	[108]

McMurray and Thorner Cell Division 2009 4:18 doi:10.1186/1747-1028-4-18

Table 1