Cell Division

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Open Access Methodology

A software solution for recording circadian oscillator features in time-lapse live cell microscopy

Daniel Sage1*, Michael Unser1, Patrick Salmon2 and Charna Dibner3

Author Affiliations

1 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

2 Department of Neurosciences, Faculty of Medicine, University of Geneva, Geneva, Switzerland

3 Division of Endocrinology, Diabetes and Nutrition, University Hospital of Geneva (HUG), Geneva, Switzerland

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Cell Division 2010, 5:17 doi:10.1186/1747-1028-5-17

Published: 6 July 2010

Additional files

Additional file 1:

Movie 1. Time lapse microscopy of Reverbα-Venus-NLS-PEST1 (Rev-VNP) protein circadian expression in NIH3T3 cells.

Format: MOV Size: 5.5MB Download file

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Open Data

Additional file 2:

Movie 2. Time lapse microscopy of Rev-VNP protein circadian expression in NIH3T3 cells transduced with LTAg SV40. The overlaid drawings (outline + trace) are displayed over frame-to-frame rescaled images.

Format: MOV Size: 5.7MB Download file

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Open Data

Additional file 3:

Movie 3. Time lapse microscopy of RFP-DNAI ligase transient expression in NIH3T3 cells. The overlaid drawings (outline + trace) are displayed over frame-to-frame rescaled images.

Format: MOV Size: 8.8MB Download file

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Open Data