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Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast

Abstract

Background

Histone post-translational modifications are critical for gene expression and cell viability. A broad spectrum of histone lysine residues have been identified in yeast that are targeted by a variety of modifying enzymes. However, the regulation and interaction of these enzymes remains relatively uncharacterized. Previously we demonstrated that deletion of either the histone acetyltransferase (HAT) GCN5 or the histone deacetylase (HDAC) HDA1 exacerbated the temperature sensitive (ts) mutant phenotype of the Anaphase Promoting Complex (APC) apc5CA allele. Here, the apc5CA mutant background is used to study a previously uncharacterized functional antagonistic genetic interaction between Gcn5 and Hda1 that is not detected in APC5 cells.

Results

Using Northerns, Westerns, reverse transcriptase PCR (rtPCR), chromatin immunoprecipitation (ChIP), and mutant phenotype suppression analysis, we observed that Hda1 and Gcn5 appear to compete for recruitment to promoters. We observed that the presence of Hda1 can partially occlude the binding of Gcn5 to the same promoter. Occlusion of Gcn5 recruitment to these promoters involved Hda1 and Tup1. Using sequential ChIP we show that Hda1 and Tup1 likely form complexes at these promoters, and that complex formation can be increased by deleting GCN5.

Conclusions

Our data suggests large Gcn5 and Hda1 containing complexes may compete for space on promoters that utilize the Ssn6/Tup1 repressor complex. We predict that in apc5CA cells the accumulation of an APC target may compensate for the loss of both GCN5 and HDA1.

Background

Eukaryotic genetic information is packaged into chromatin, a highly organized and dynamic protein-DNA complex. The fundamental unit of chromatin, the nucleosome, is an octameric structure composed of two copies of each of the four core histones (an H3/H4 tetramer and two H2A/H2B dimers), surrounded by approximately 146 bp of DNA [1, 2]. Many cellular processes depend on modifications of both DNA and histones within nucleosomes [3, 4]. Modification of chromatin by histone acetyltransferases (HATs) and histone deacetylases (HDACs) play key roles in transcriptional regulation [5–9]. Post-translational acetylation of the highly conserved lysines within the N-terminal tail domains of the core histones is strongly correlated with transcriptional activation [5, 10]. Although the precise mechanisms by which histone acetylation alters transcription are poorly understood [9–12], there is tremendous pressure to understand these mechanisms, as impaired histone modification is linked to many disease states [13].

The study of HAT and HDAC recruitment to promoters and their interaction with activators and repressors are essential for a better understanding of gene regulation. HATs and HDACs modify histones enzymatically throughout the genome [14]. Histone acetylation potentially regulates transcription by manipulating the higher-order folding properties of the chromatin fiber [15–17]. General control nonderepressible 5 (Gcn5) [18] was the first identified HAT and exists as the catalytic subunit in multiple high molecular weight complexes in yeast, including SAGA (Spt-Ada-Gcn5-Acetyltransferase), SLIK (SAGA-like), ADA (transcriptional ADAaptor), and the smaller HAT-A2 complex [19–23]. As part of the evolutionarily conserved SAGA complex, Gcn5 predominantly acetylates nucleosomal H3 lysines K9, K18, and K27 [24]. Defects in human SAGA subunits are associated with multiple disorders, including neurological diseases and aggressive cancers [25, 26]. Gcn5 is a direct target for recruitment by transcriptional activators in vitro [27, 28] and in vivo [29], which results in the acetylation of nearby histones [10]. Elongation of the transcripts initiated by Gcn5-containing complexes is carried out by the Elongator complex, which utilizes Elp3 as its primary HAT [30, 31]. Cell cycle specific roles for Gcn5 have been reported, as recruitment of Gcn5 to a set of genes that are expressed in late mitosis requires SWI/SNF remodelling activity [32]. Furthermore, Gcn5 displays an overlapping pattern of localization with several HDACs [24, 33, 34]. Acetylation microarrays have shown that Rpd3 and Hda1 are the principal HDACs in yeast, affecting numerous promoters throughout the genome with little overlap between promoters [10, 35]. Hda1, an evolutionary conserved HDAC, which deacetylates mainly histones H2B and H3 [36, 37], is recruited to promoters via utilization of different Tup1/Ssn6 domains [38–40], resulting in local deacetylation. HDAC recruitment may form a positive feedback loop to repress transcription locally and facilitate the spreading of Tup1 into adjacent regions [41]. Tup1-mediated repression requires the deacetylation of histones within promoters [42–44], which may require direct recruitment of HDACs [36, 45, 46]. Overall, the mechanisms of Tup1/Ssn6-mediated transcriptional repression can be classified into 3 classes: (i) direct interaction with the activator; (ii) repression by changing chromatin structure; and (iii) interaction with the general transcription machinery [47, 48]. It appears that different groups of genes have developed different strategies to utilize Tup1/Ssn6, enabling it to function as a global repressor.

Our work has linked the Anaphase Promoting Complex (APC), an evolutionarily conserved 13 subunit complex in yeast that is critical for mitotic progression and G1 maintenance [49–52], with chromatin assembly and histone acetylation through genetic interactions with chromatin assembly factor (CAF), HAT and HDAC mutants [53–57]. The APC is a ubiquitin-protein ligase (E3) that targets proteins that block the initiation of anaphase (Pds1) and mitotic exit (Clb2) for degradation. Various regulators govern APC activity in positive and negative manners, from phosphorylation and transcriptional control of APC subunits, to sequestration of APC activators [58–63]. For example, protein kinase A (a complex of Bcy1, Tpk1, Tpk2 and Tpk3) and Mad2 inhibit APC activity through phosphorylation and subunit sequestration, respectively. Activating phosphorylation is supplied by the polo-like kinase (Cdc5) and Cdc28. Furthermore, Cdc20, inhibited by a Mad2-dependent mechanism, binds and activates the APC to promote the metaphase/anaphase transition, while Cdh1, another APC-binding partner, drives APC-dependent mitotic exit. Previous studies by our group have expanded the APC's functional repertoire by showing that the mutant APC subunit allele, apc5CA [54], genetically interacted with deletions of the HAT encoding genes GCN5 and ELP3 [57]. Strains harboring the apc5CA gcn5 Δ or the apc5CA elp3 Δ mutations had severely restricted growth at elevated temperatures compared to the single mutants. This interaction implies that the APC and these HATs positively interact, but a negative feedback loop appears apparent, as G1-specific Gcn5 instability was reduced in APC mutant cells. An additional synergistic genetic interaction between hda1 Δ and apc5CA was also observed, suggesting that the APC interacts positively with the HDAC Hda1 [57]. The study presented here focuses on a novel antagonistic relationship between gcn5 Δ and hda1 Δ that is revealed in apc5CA , but not APC5 cells. We provide further evidence that the APC works with multiple histone modifiers to drive cell cycle progression.

Results

gcn5 Δ/hda1 Δ interactions revealed in an APC mutant background

In a recent screen, we identified HAT (gcn5 Δ) and HDAC (hda1 Δ) deletions that severely impacted the apc5CA (c hromatin a ssembly defective) [54, 57] temperature sensitive (ts) phenotype, indicating that both proteins have a positive influence on Anaphase Promoting Complex (APC) activity. The apc5CA mutation was identified in a chromatin assembly mutant screen; the allele contains an AT deletion altering amino acid 12, which created an in-frame stop codon 12 amino acids further along [54]. We recently observed that the apc5CA-TAP (Tandem Affinity Protein purification) protein migrates faster, with less intensity, than the wild type Apc5-TAP by SDS-PAGE, indicating that apc5CA encodes an N-terminal truncation (data not shown). Here we show that deletion of HDA1 in gcn5 Δ cells had no apparent effect (Figure 1A), whereas deletion of HDA1 in apc5CA gcn5 Δ cells improved ts growth. The apc5CA background therefore allowed the study of a previously uncharacterized antagonistic interaction in yeast between Gcn5 and Hda1. Plant GCN5 was also found to interact antagonistically with HD1, the Hda1 orthologue, to regulate light-responsive gene expression [64], but mechanisms remained undetermined.

Figure 1
figure 1

Mutation to the APC subunit Apc5 reveals antagonistic interactions between Gcn5 and Hda1. (A) Serial 10-fold dilutions of each strain were spotted onto YPD plates from left to right and incubated at the temperatures shown. (B) Serial dilutions using strains expressing the indicated plasmids were spotted onto SD-ura plates containing either 2% glucose or 2% galactose, and grown at 30°C for 2 and 3 days, respectively. (C) Protein lysates were prepared from the mutants shown and characterized by Westerns using the antibodies indicated. Antibodies against GAPDH were used as load controls.

To examine whether Hda1 positively interacted with the APC, we expressed galactose driven HDA1 carrying a C-terminal HA tag (GAL pro HDA1-HA) at low levels in WT, apc5CA and gcn5 Δ cells by using glucose as a carbon source (Figure 1B). Recently, we observed that mRNA levels of GAL pro GCN5-HA were elevated 100-fold when grown on 2% glucose and 900-fold when grown on 2% galactose [57]. However, Gcn5-HA protein expression remained low even though GCN5-HA mRNA was 100-fold elevated when grown on 2% glucose. As shown with GCN5 [57], low-level GAL pro HDA1-HA expression improved apc5CA growth (Figure 1B). This is not necessarily a general feature of histone modifying proteins, as deletion or overexpression of the HAT HPA2 had little effect on apc5CA cells (Figure 1B) [57]. Although the yeast Hpa2 has not yet been shown to acetylate histones in vivo, a bacterial acetyltransferase that does acetylate eukaryotic histones is most closely related to Hpa2, and Hpa2 does acetylate H3 in vitro [65, 66]. Moreover, Hpa2 appears to be active, as overexpression reduces growth of gcn5 Δ cells, whereas expression on glucose improves growth of apc5CA cells (Figure 1B).

A further connection between Gcn5 and Apc5 was observed by the rescue of GAL pro APC5-HA overexpression toxicity by deletion of GCN5 (Figure 1B). It is unlikely that Apc5 protein levels induced from the GAL promoter are compromised in gcn5 Δ cells, as expression of HPA2 and HDA1 from the GAL promoter reduces gcn5 Δ growth. Overexpression of APC5 from the CUP1 promoter also reduced yeast replicative lifespan [60]. Rescue of APC5 toxicity by GCN5 deletion is consistent with our recently proposed hypothesis that Gcn5 is required for APC activity, and may provide an explanation as to why GCN5 [57] and HDA1 (Figure 1B) overexpression is toxic, considering that overabundance of Apc5 is detrimental to cells.

Next, we asked whether mutations to APC5 influenced acetylation of histone H3 lysine 9 or 14 (H3K9/14) in gcn5 Δ and hda1 Δ cells. Gcn5 appears to play a greater role on H3K9, compared to H3K14, whereas loss of HDA1 results in increased acetylation of both H3K9 and H3K14 (Figure 1C). The apc5CA background did not change the acetylation status of H3K9/14 in gcn5 Δ or hda1 Δ cells, suggesting the apc5CA background may be revealing an effect other than global histone H3 acetylation. H3K9Ac was reduced in gcn5 Δ, apc5CA gcn5 Δ and apc5CA gcn5 Δ hda1 Δ cells, but not in gcn5 Δ hda1 Δ cells. The ability to acetylate H3K9 in gcn5 Δ hda1 Δ cells indicates that on a global level, other HATs can use H3K9 as a substrate. However, at the gene level, deletion of GCN5 was previously shown to reverse histone hyperacetylation at the PHO5 promoter when HDA1 was deleted [67]. Therefore, we tested whether transcript levels are influenced by apc5CA in gcn5 Δ or hda1 Δ cells.

The apc5CAallele increases transcript levels in hda1 Δ cells

Since the apc5CA allele had little effect on global histone H3K9/14 acetylation, we asked whether individual gene transcripts were altered. We chose to study several genes involved in APC function, as altered expression of APC regulators may underlie the observed growth phenotypes. Thus, we performed Northern and reverse transcriptase PCR (rtPCR) experiments to determine expression of CDC20, PDS1, BCY1 and MAD2. Cdc20 plays a positive role in APC activity, whereas Pds1, Bcy1 and Mad2 have a negative impact [58–63]. Northerns (Figure 2A) and rtPCR (data not shown) both show that compared to RDN1, expression of the tested transcripts were reduced in gcn5 Δ cells, especially at 37°C, whereas transcripts in hda1 Δ cells were relatively unimpaired. The bands from 2 Northerns and 2 rtPCR experiments were scanned, quantified and averaged, with the expression of each gene for each experiment normalized to RDN1. This number is relative to expression in the wild type strain, which was set to 1 (Figures 2B, C). Although previous microarray analyses in gcn5 Δ and hda1 Δ cells did not identify these genes [68, 69], the approximate 2-fold decrease in transcript levels in gcn5 Δ cells (Figure 2C) suggests Gcn5 is involved in expression of the tested genes. However, while the apc5CA allele had no apparent effect on transcript levels in gcn5 Δ cells, in apc5CA hda1 Δ cells, BCY1 transcripts (Figure 2B) and PDS1 transcripts (Figure 2C) were clearly elevated. The loss of this effect in the triple mutant suggests Gcn5 may be required for elevated transcription in apc5CA hda1 Δ cells.

Figure 2
figure 2

Expression of PDS1 , an APC antagonist, is specifically elevated in apc5CA hda1 Δ cells at 37°C. (A) Northern analyses were conducted on total RNAs extracted from the various mutants and probed with sequences derived from the coding regions of the genes indicated. RDN37-2 (RDN1) was used as a loading control. All bands from the 30°C experiments (B) and the 37°C experiments (C) were quantified by ImageJ and normalized to the RDN1 signal. Densitometry was performed on two Northern experiments and two reverse transcriptase experiments. The data was combined and the means and standard errors were plotted.

Increased PDS1 transcripts in apc5CA hda1 Δ cells correlates with increased promoter acetylation

Our data suggests the apc5CA allele enhances the transcript levels of some of the tested genes in hda1 Δ cells. We next tested whether promoter acetylation of these genes was similarly impacted using chromatin immunoprecipitation (ChIP) with antibodies that recognized acetylated lysines 9 and 14 on histone H3 (H3K9/14Ac), and primers that amplified 200 basepair fragments immediately upstream of the transcriptional start site of the genes studied above. We used H3K9/K14Ac antibodies to capture acetylation of both H3K9 and H3K14 as our studies show these residues are targeted by Gcn5 and Hda1. We assessed promoter acetylation in gcn5 Δ, hda1 Δ and gcn5 Δ hda Δ1 mutants in the apc5CA background (Figure 3A). Antibodies against total H3 and a no antibody mock treatment were used as controls. The bands in all experiments were quantified and analyzed (Figure 3B). Once background densities were subtracted from all bands, the H3K9/14Ac/total H3 ratio was determined. The values represent two independent experiments, as described previously [66, 70]. H3 promoter acetylation was reduced in both apc5CA gcn5 Δ and apc5CA gcn5 hda1 Δ cells, similar to the transcript patterns at 37°C (Figure 2C), strengthening the notion that Gcn5 HAT activity is tightly correlated with transcription. However, it is interesting to note that while promoter acetylation is equally low in apc5CA gcn5 Δ cells at 30 and 37°C, transcript defects are only obvious at 37°C. Notably, a previous study observed that Gcn5-dependent transcription and promoter histone acetylation activities could be uncoupled [71].

Figure 3
figure 3

Histone H3 acetylation at promoter regions is elevated specifically in apc5CA hda1 Δ cells at 37°C. (A) ChIP was performed using lysates derived from the mutants shown and antibodies against total H3 or H3 acetylated at both K9 and K14. A mock treatment lacking antibody was used as a control. Once the crosslinks were reversed and DNA recovered, "end point" PCR was performed using primers against the genes shown that amplified 200 bp regions of the promoter. 10% of the reaction was used as input. (B) Two independent experiments were quantified, with the means and standard errors represented graphically, as previously described [66, 70].

Consistent with our observations that transcript levels of BCY1 and PDS1 increase in apc5CA hda1 Δ cells, we detected increased BCY1 and PDS1 promoter acetylation in these cells, specifically at 37°C. Transcript levels and promoter acetylation are both increased with PDS1 at 37°C in apc5CA hda1 Δ cells. However, we note some differences in the patterns observed. For example, BCY1 transcripts are not elevated in apc5CA hda1 Δ cells at 37°C while promoter acetylation is. This may reflect the complex nature of the factors assembled at promoters that is not addressed in this study.

Gcn5 promoter occupancy increases in the absence of Hda1

One possible scenario to explain increased PDS1 promoter acetylation and transcription in apc5CA hda1 Δ cells may be increased availability of Gcn5 or related HATs due to the apc5CA allele. It was previously reported that in cells expressing defective TUP1, increased Gcn5 was observed at Tup1-repressible promoters, thereby derepressing transcription [40]. We have speculated that the APC may target Gcn5 for turnover in order to progress through the G1/S transition [57]. To examine this possibility, endogenous GCN5 was TAP-tagged in WT, apc5CA and apc10 Δ cells and detected by Westerns in asynchronous early log phase cells. Gcn5 protein levels were indeed increased in both apc5CA and apc10 Δ cells (Figure 4A). While this does not explain the genetic interaction between gcn5 Δ and hda1 Δ in apc5CA cells, it does suggest the possibility that a factor related to Gcn5 may also be elevated in apc5CA cells. Consistent with this hypothesis, we observed increased Gcn5-HA and Elp3-HA, expressed from the GAL promoter, in cells lacking the proteasome ubiquitin receptor Rpn10 (Figure 4B). As controls, we TAP-tagged APC5 in rpn10 Δ cells, which was unaffected by rpn10 Δ. Furthermore, GAPDH was also unaffected by rpn10 Δ, whereas ubiquitinated proteins did accumulate. Therefore, in cells lacking a functional ubiquitin system, at least Gcn5 and Elp3 accumulate.

Figure 4
figure 4

Deletion of HDA1 results in increased Gcn5 at promoters. (A) Steady-state Gcn5-TAP in different mutant backgrounds was determined in early log phase asynchronous cells grown at 30°C by Western blotting. Westerns were performed using antibody against TAP and the membrane was stained with Ponceau S to confirm equal protein load. (B) Plasmid borne HA-tagged GCN5 and ELP3, driven by the galactose inducible promoter, were expressed in cells lacking the proteasome ubiquitin receptor Rpn10. Cells were grown overnight in 2% glucose to early log phase. The glucose-supplemented media was washed away and the cells were resuspended in 2% galactose-supplemented media. The cells were then split with one half incubated at 37°C and the other half left at 30°C. The cells were incubated for an additional 4 hours, afterwhich proteins were harvested and analyzed with antibodies against HA or GAPDH as a load control. Controls for the experiment included endogenous APC5-TAP in rpn10 Δ cells, as well as the detection of endogenous Clb2 and Ub in WT and rpn10 Δ cells using commercially available antibodies. (C) Protein/DNA complexes were recovered from the mutants shown following GAL-induction using antibodies against either the HA epitope, total H3, or H3K9/14Ac. A mock treatment was conducted where antibody was omitted. Recovered DNA was used as template in "end point" PCR reactions using primers that amplified the promoter regions indicated. 10 μl of each reaction was separated by agarose gel electrophoresis and scanned. (D) Two independent experiments were performed. The gels were scanned and quantified using ImageJ. The means and standard errors were plotted.

Next we asked whether promoter occupancy by Gcn5 correlated with gene expression and promoter acetylation. GAL pro GCN5-HA was induced in gcn5 Δ and gcn5 Δ hda1 Δ cells so that the only Gcn5 expressed was HA tagged. gcn5 Δ cells expressing GAL pro GCN5-HA grew like WT (data not shown), and were considered the WT control for this experiment. ChIP was performed in lysates prepared from these cells. Control ChIPs were performed using untagged lysates (data not shown), and reactions without antibody, neither of which produced PCR products. Primers against the 5', middle, and 3' regions of CDC20 demonstrated that Gcn5-HA recruitment was most prominent at the promoter and was reduced 5' to 3' (data not shown). We found that in HDA1 cells expressing GCN5-HA, very little Gcn5-HA was present at the promoters tested compared with the RDN1 promoter (Figures 4C and 4D). In hda1 Δ GCN5-HA cells, however, increased Gcn5-HA promoter recruitment was observed. The increases observed were slight except for the CDC20 promoter. Promoter acetylation also increased in hda1 Δ cells, consistent with increased recruitment of Gcn5. These observations present the possibility that i) increased promoter H3K9/14 acetylation in hda1 Δ cells is due to increased Gcn5-HA promoter recruitment; and/or ii) Hda1 may block access of Gcn5 to promoters.

It is possible that increased Gcn5-HA recruitment is due to increased GAL pro -driven Gcn5 expression in hda1 Δ cells, since Hda1 represses galactose-induced gene activation [72]. We assessed expression of Gcn5-HA in the strains used above and observed that GAL pro GCN5-HA expression after a 5 hour induction period was reduced in hda1 Δ cells (Figure 5A). Therefore, it is unlikely that the decreased levels of GAL-promoter driven GCN5 in hda1 Δ cells are due to Hda1's influence on the GAL promoter. Considering that hda1 Δ cells express less GCN5 than WT, yet recruit a greater amount of Gcn5 to promoters, a much greater proportion of Gcn5-HA must be available for recruitment in hda1 Δ cells. To examine this possibility, we performed ChIP using lysates prepared from GAL pro GCN5-HA expressing cells after 1, 3 and 5 hours of induction (Figure 5B). Gcn5-HA was recruited to each tested promoter (Figures 5C and 5D). When normalized to input, Gcn5-HA recruitment in HDA1 cells was similar at each induction timepoint (Figure 5D). In hda1 Δ cells however, Gcn5-HA recruitment was again increased, and recruitment increased the longer the induction. Together, our data suggests that in the absence of Hda1, Gcn5-HA continually gains access to the tested promoters.

Figure 5
figure 5

Gcn5 promoter occupancy is kept at an equilibrium in WT cells, but increases over time in hda1 cells. (A) Western analyses of Gcn5-HA expression in gcn5 Δ and gcn5 Δ hda1 Δ cells following a 5 hour 4% galactose-induction. Antibodies against GAPDH were used as a load control. (B) A galactose-induction time-course was performed in gcn5 Δ and gcn5 Δ hda1 Δ cells expressing GAL pro -GCN5-HA. Protein samples were removed at the times shown for Western analyses with antibodies against HA and GAPDH. (C) From the time-course described above, samples were also removed for ChIP. Recovered DNA was used as a template in "end point" PCR reactions. S, sample with antibody; C, control without antibody; I, 10% lysate input. (D) The gel in (C) was scanned, analyzed using ImageJ and plotted.

Tup1 occludes Gcn5 promoter occupancy

We next tested whether the impact of Hda1 on Gcn5 promoter accessibility involved the corepressor complex Ssn6/Tup1. Several reports have demonstrated that the Ssn6/Tup1 corepressor utilizes Hda1 to repress transcription of target genes [36, 41, 68]. Furthermore, Tup1 has been shown to recruit Gcn5 to repressed promoters [73–75]. It was proposed that this may set the stage for derepression of silent genes. Thus, GAL pro GCN5-HA was induced in hda1 Δ and tup1 Δ cells as the only source of Gcn5, followed by ChIP. Gcn5-HA expression in hda1 Δ cells was reduced compared to WT, but expression in tup1 Δ cells was unchanged (data not shown). We found that in otherwise WT strains (gcn5 Δ + GALproGCN5-HA), Gcn5-HA was weakly recruited to the tested promoters (Figures 6A and 6B). In strains lacking HDA1 or TUP1, Gcn5-HA promoter occupancy was observed to increase. We also observed that in cells lacking SSN6, promoter recruitment of Gcn5-HA increased (data not shown). These results suggest that Hda1 may work together with the Ssn6/Tup1 corepressor complex to impede access of Gcn5 to the tested promoters. However, Hda1 and Gcn5 may also compete for Tup1 interactions. It is also feasible that Tup1 utilizes different mechanisms to reduce Gcn5 promoter occupancy.

Figure 6
figure 6

Tup1 occludes Gcn5 recruitment. A) ChIP was performed using the cells shown expressing GAL pro -GCN5-HA following a 5 hour galactose induction, as described above. (B) Two independent experiments were scanned and processed using ImageJ, with the means and standard errors shown. (C) Strains lacking TUP1 were constructed in WT and apc5CA backgrounds. Growth phenotypes were assessed by spot-dilutions, followed by incubation at 34°C and 37°C.

To distinguish between these possibilities, we predicted that if Tup1 and Hda1 work together, then deletion of TUP1 in apc5CA cells should have the same synergistic effects as an HDA1 deletion. Our results show that deletion of TUP1 impairs the apc5CA phenotype (Figure 6C), similar to an hda1 Δ mutation. This suggests that both Hda1 and Tup1 perform a function that is beneficial to APC activity. However, it does not necessarily indicate they work together to perform this task.

Hda1 and Tup1 likely interact at promoters, which can be inhibited by Gcn5

Others have also shown Tup1 and Hda1 functionally interact to repress gene transcription [36, 41, 68], and to associate in vitro [36], but not necessarily in vivo [46]. To investigate whether Tup1 and Hda1 do function together, we asked if Tup1 and Hda1 can physically interact at promoters, and if Gcn5 can influence this. To do so we performed sequential ChIP in cells expressing a combination of Hda1-HA and/or GST-Tup1. ChIP was first performed using antibodies against HA. Bound proteins were released, recovered, and incubated with antibodies against GST. Bound protein/DNA complexes were again isolated and PCR was performed using primers against the test promoters. The results show that in cells expressing either Hda1-HA or GST-Tup1, no bound DNA was recovered (Figure 7A and 7B). However, in cells co-expressing the plasmids, PCR fragments were obtained for all promoters tested. This supports the idea that Tup1 and Hda1 can associate in vivo at specific promoters. Nonetheless, this could also reflect close, but independent Hda1 and Tup1 binding on the same promoter. In gcn5 Δ cells co-expressing the plasmids, putative complex formation was again observed, and was visibly increased at CDC20, PDS1, and BCY1 promoters, suggesting Gcn5 may negatively impact this interaction. Figure 7C shows that the proteins were expressed similarly in the strains used. Our experiments do not differentiate between Hda1 and Tup1 physically binding, or whether they simply bind adjacent DNA sequences, but it is important to note Hda1 and Tup1 were previously shown to physically associate [36], and that the interaction observed by sequential ChIP is enhanced by GCN5 deletion.

Figure 7
figure 7

Gcn5 can inhibit Hda1-Tup1 associations at some promoters. (A) Sequential ChIP was used to observe Hda1-Tup1 physical interactions at specific promoters. WT, hda1 Δ and gcn5 Δ hda1 Δ cells expressing combinations of GAL pro -HDA1-HA and CUP1 pro -TUP1-GST were induced using 4% galactose for 5 hours and 0.4 mM CuSO4 for 3 hours. ChIP reactions were first performed with antibodies against HA. Bound proteins were eluted from beads using 10 mM DTT for 30 minutes at 37°C. The eluted proteins were then incubated with anti-GST antibodies. The immune complexes were isolated again, cross links were reversed, and "end point" PCR was performed using the recovered DNA as template. (B) Two independent experiments were performed and processed using ImageJ. The means and standard errors are shown. (C) Westerns showing expression of the proteins used in the sequential ChIP experiment.

Taken together, the results presented in this report suggest a competitive interaction can occur between Hda1/Tup1 and Gcn5 at promoters (Figure 8). Our results suggest that the presence of Hda1/Tup1 (and likely Ssn6) occludes, at least partially, the recruitment of Gcn5 to some promoters. Gcn5, on the other hand, may impede Tup1-Hda1 interactions by competing for Tup1 binding. It is possible that the gcn5 Δ/hda1 Δ genetic interaction is prominent in apc5CA cells due to the accumulation of an APC target, perhaps another HAT, capable of suppressing gcn5 hda1 Δ impairments.

Figure 8
figure 8

A model depicting potential interactions between Gcn5 and Hda1. (A) The HAT Gcn5 and the HDAC Hda1 have opposing functions that individually benefit APC function. (B) and (C) Gcn5 and Hda1 appear to compete for Tup1 binding. (B) If Hda1 first gains access to the promoter, recruitment of Gcn5 is partially blocked. (C) Under conditions where gene transcription must be derepressed, Tup1 may recruit Gcn5 to the promoter to prime transcriptional initiation, thus displacing Hda1. The protein labelled × represents a DNA binding factor that recruits the Tup1/Ssn6 corepressor complex to silent genes.

Discussion

Novel Gcn5/Hda1 antagonistic functional interactions are revealed when APC activity is compromised

The work presented here provides evidence to support a model in which the HAT Gcn5 and the HDAC Hda1 functionally interact at promoters to determine transcriptional readouts (Figure 8). In otherwise WT cells, mutations to GCN5 or HDA1 do not create significant growth defects, whereas in apc5CA cells, these same mutations produce severe ts growth defects (Figure 1A). The focus of this study was to characterize an antagonistic functional gcn5 Δ/hda1 Δ interaction revealed in the apc5CA background, as the severe apc5CA gcn5 Δ and apc5CA hda1 Δ ts defects are suppressed in apc5CA gcn5 Δ hda1 Δ cells. Growth phenotypes associated with deletion of GCN5 have been shown in two separate Synthetic Genetic Array (SGA) genome-wide screens to be suppressed by deletion of HDA1 [76, 77]. However, spot dilution analysis of the gcn5 Δ and hda1 Δ cells on YPD did not reveal any phenotypes [76], as shown in our study (Figure 1A). Thus, the gcn5 Δ hda1 Δ antagonistic interaction is not apparent under normal growth conditions, such as on YPD, but under conditions imposed by the SGA screen (selective media, for example), the antagonistic interaction can be exposed. The influence of the apc5CA allele on this interaction was investigated. The apc5CA allele had little effect on global histone H3 acetylation status in gcn5 Δ and hda1 Δ cells, but did cause the increase of BCY1 and PDS1 transcripts in hda1 Δ cells (Figures 1C, 3). Both Bcy1 and Pds1 proteins antagonize APC activity and may be involved in the enhanced growth defect when APC is mutated. Therefore, in apc5CA cells, it may be the inappropriate expression of inhibitory transcripts that are paramount to synergistic apc5CA gcn5 Δ and apc5CA hda1 Δ phenotypes.

A molecular mechanism explaining the Gcn5/Hda1 interaction likely involves competition for Tup1 binding. We observed that in cells lacking HDA1 or TUP1, Gcn5 recruitment at our tested promoters was increased (Figures 4 and 6). On the other hand, deletion of GCN5 increased Hda1-Tup1 physical interactions at promoters (Figure 7). A competition between Hda1 and Gcn5 for Tup1 binding is a possibility worth considering, as both Hda1 and Gcn5 have been shown to physically interact with Tup1 [36, 73–75]. However, in gcn5 hda1 Δ cells this mechanism would not be possible. In addition to the accumulation of Gcn5 in apc5CA cells, we observed that Elp3 also accumulates when the ubiquitin system is compromised (Figures 4A, B). We previously demonstrated that gcn5 Δ and elp3 Δ deletions impair apc5CA defects, that GCN5 and ELP3 overexpression stalls the cell cycle in G1, and that Gcn5 G1-specific instability is reversed in APC mutants [57]. Thus, when apc5CA is combined with gcn5 Δ hda1Δ, an APC target likely accumulates that creates novel transcripts that allow bypass of the severe ts defects observed in the double mutants. Elp3 is an attractive candidate since it is involved in elongating transcripts initiated by Gcn5 containing complexes [31]. A global transcript analysis is likely required to follow this further. Our previous work suggests that the apc5CA phenotype is sensitive to global transcript levels [57].

Hda1-dependent occlusion of Gcn5 from promoters requires Tup1

Several reports describe the recruitment of the Tup1/Ssn6 repressor complex to DNA via interactions with multiple partners [41, 48, 68]. Once recruited, Tup1 then contacts H3 and H4 N-terminal tails [78]. Mechanisms employed to recruit Tup1/Ssn6 to promoters by the various individual interacting partners appears to be complex, seems to vary, and may have overlapping roles. Gcn5-HA recruitment to the tested promoters was increased in hda1 Δ, tup1 Δ and ssn6 Δ cells (Figure 6; data not shown), indicating that the interaction of Hda1 with the Tup1/Ssn6 repressor complex is necessary to block access to Gcn5. Tup1 and Hda1 did indeed co-immunoprecipitate while bound to the same promoters, as shown by sequential ChIP (Figure 7). We find it unlikely that Tup1 and Hda1 are simply associating independently at adjacent sequences within the 200-basepair DNA PCR fragment, since they have been shown to interact previously [34], and are part of large complexes [19–23], but we cannot discount this possibility. However, we observed that in gcn5 Δ cells, Hda1-Tup1 association increased at some promoters (PDS1 and BCY1), suggesting Gcn5 opposes complex formation. The mechanism of action that Gcn5 uses to block Hda1-Tup1 association remains unclear. Previous reports indicating that Tup1 is capable of recruiting and interacting with Gcn5/SAGA at promoters [73–75] suggest it is possible that Gcn5 and Hda1 may compete for Tup1 interaction. The scenario for recruiting either Gcn5 or Hda1 would differ, implying other proteins may be involved in deciding whether Gcn5 or Hda1 gain access. We were unable to observe complex formation between Gcn5-TAP and Hda1-HA in whole cell lysates (data not shown), indicating possible exchange of Gcn5 and Hda1 at Tup1 complexes does not require Gcn5-Hda1 association. It is also possible that Gcn5-Hda1 physical interactions are transient and promoter specific, therefore may not be detectable using the methods applied here. Nonetheless, support for our model was provided by reports describing recruitment of Gcn5 to promoters by the Tup1/Ssn6 complex under osmotic stress conditions [40, 74], indicating that Tup1/Ssn6 may be a transcriptional activator under certain conditions.

Conclusions

The results presented in this manuscript provide evidence for a complex network of interactions between a mitotic/G1 cell cycle regulator (the APC), and antagonistic interplay between a HAT (Gcn5), and an HDAC (Hda1). Gcn5 is known to function during mitosis [32, 57, 79, 80]. Data on the role Hda1 plays in cell cycle progression is limited, but Hda1 may provide some function to ensure histones are deacetylated prior to passage through mitosis [81]. It is noteworthy that Gcn5 and Hda1 expression is temporally regulated during the cell cycle (microarray data compiled at Saccharomyces Genome Database), providing insight into how the potential competition for Tup1 binding could be regulated. APC mutations cause cell cycle progression to stall during mitosis, potentially skewing the equilibrium between Gcn5 and Hda1 promoter recruitment if the cell cycle does indeed influence Hda1 and Gcn5 recruitment. Future work will focus on identifying the molecular mechanisms regulating how cell cycle progression influences chromatin dynamics. Chromosome synthesis and segregation defects are widely associated with human disease, thus continued work into furthering our understanding of this process is vital.

Methods

Media, yeast strains, plasmids and general methods

Cells were grown in YPD (1% yeast extract, 2% peptone, 2% glucose) or synthetic complete drop-out media (SD; 0.17% yeast nitrogen base, 0.5% (NH4)2SO4, 2% glucose [or 4% galactose], 1.3 g amino acid drop-out powder/1 L, 1 tablet NaOH). Genes under the control of the galactose promoter were induced with 4% galactose for 5 hours. All yeast strains were S288c derivatives unless mentioned otherwise (Table 1). Double and triple mutants were created by crossing appropriate strains, followed by multiple rounds of backcrossing. The strains used here were considered congenic. Some mutants, such as tup1 Δ (YTH3922), were created by one-step homologous recombination as previously described [54]. Primers flanking the TUP1 ORF by 500 basepairs were used in PCR reaction with genomic DNA from YTH1449 as template. Colonies that grew on Geneticin (G418) were confirmed by PCR. GCN5 was TAP-tagged on the C-terminus using one-step homologous recombination. Primers designed to flank the GCN5 stop codon by 500 basepairs on either side were used in PRC reactions with genomic DNA isolated from YTH3864 as template. PCR fragments were then transformed into YTH1235 cells. Colonies that formed on SD-his plates were confirmed by PCR and Western analyses. Plasmids and sources used in this study are provided in Table 2. Yeast and E. coli transformations were done according to published procedures [54]. Overexpression from the CUP1 promoter was accomplished by adding 0.4 mM CuSO4 to liquid growth media for 3 hours. Spot dilutions were performed by determining the OD600 of overnight cultures and then diluting the cells to 5 × 107/ml. Ten-fold serial dilutions were prepared, with 3 μl volumes of each dilution spotted onto the appropriate media and incubated at a variety of temperatures. Northerns and Westerns were performed as described previously [54, 60]. Primers used in the Northern analyses are shown in Table 3. Rabbit polyclonal anti-H3K9Ac (Upstate Biotechnology), rabbit monoclonal anti-H3K14Ac (Abcam), rabbit polyclonal anti-H3 (Abcam), rabbit polyclonal anti-HA (Abcam), and rabbit polyclonal anti-GST (Abcam) were used at 1:4000. Rabbit polyclonal anti-Clb2 (Santa Cruz; Y-180) and mouse monoclonal anti-ubiquitin (Cell Signalling Technology; P4D1) were used at 1:2000. The TAP antibody (Open Biosystems) was used at a dilution of 1:1000. Mouse monoclonal anti-GAPDH (Sigma) was used at a dilution of 1:20,000. Horseradish peroxidase (HRP)-conjugated secondary antibodies were used at a dilution of 1:20,000 for GAPDH, and for all other antibodies 1:10,000, and detected by enhanced chemiluminescene (PerkinElmer).

Table 1 Yeast strains used in this study
Table 2 Plasmids used in this study
Table 3 Primers generated for the Northern analysis

Reverse transcriptase PCR (rtPCR)

Total RNA was treated with RNase-free DNase (Fermentas Life Sciences) following the manufacturer's recommendations. 1-5 μg of total RNA was used for cDNA synthesis using an oligo(dT) primer and M-MLV reverse transcriptase (Fermentas). RNA was incubated at 70°C for 10 minutes prior to the reverse transcriptase reaction. Finally, 1 μl of each cDNA sample was used as template in PCR reactions with the primers described in Table 4 to amplify each of the target messages. To determine the PCR linear range for each message, 50 μl PCR reactions were prepared using WT cDNA with each primer set (Table 3). 5 μl of each reaction was removed every 2 cycles, analyzed using 1% agarose gel electrophoresis, and stained with ethidium bromide (data not shown). The gel was scanned and ImageJ was used to determine the mid-linear range cycle for each reaction. Subsequent rtPCR reactions were set up to cycle only to the predetermined mid-linear range. Primers that amplified the noncoding 18S rRNA RDN37-2, which is within the RDN1 locus (referred to as RDN1), were designed to generate a fragment for use as a control in Northerns and rtPCR.

Table 4 Primers generated for the ChIP analysis

Chromatin immunoprecipitation (ChIP)

ChIP was performed essentially as described elsewhere [82, 83] with the following modifications: DNA fragment size achieved by sonication was 500-1000 bp, and 100 μg of protein lysate was used for each IP. Protein concentration was determined by a Bradford protein assay. 5 μg of ChIP grade rabbit polyclonal anti-acetyl-H3K9/14 (Upstate Biotechnology), rabbit polyclonal anti-H3 (Abcam), rabbit polyclonal HA antibody (Abcam), and rabbit polyclonal GST antibody (Abcam) were used for IP. One-tenth of the total volume of lysate was used as input for each sample. Sequential ChIP was performed as previously described [84]. In sequential ChIP experiements, the immune complexes were eluted by incubation for 30 minutes at 37°C in 10 mM DTT. After centrifugation, the supernatant was diluted 25 times with ChIP dilution buffer (1% Triton X-100, 2 mM EDTA, 150 mM NaCl, 20 mM Tris-HCl [pH 8.1]) and subjected again to ChIP using a different antibody. In this experiment, HA antibody was applied first, followed by GST antibody. Cross-linking of the immune complex was reversed by adding NaCl to a final concentration of 0.3 M and incubated overnight at 65°C. Samples were treated first with 1 μg/μl RNaseA (Millipore [formerly Upstate]) for 30 minutes at 37°C, followed by 1 μg/μl proteinase K (Millipore [formerly Upstate]) at 45°C for 1 hour. DNA was purified by chromatography on QIAquick columns, and eluted with elution buffer (PCR purification kit, Qiagen). PCR was performed for semiquantitative determination by standard end point PCR. 1 μl DNA was used for PCR, and the reaction continued to the predetermined mid-linear range for each primer set. The end point PCR product was resolved on a 1% agarose gel and visualized by ethidium bromide. Two independent experiments were performed for each ChIP. The gel bands from each experiment were analyzed by ImageJ, and the means and standard error were plotted for graphical representation. For time course experiments, 200 ml cultures were induced at a final concentration of 4% galactose. Samples (20 ml) were immediately removed, and again after 1, 3 and 5 hours. The 20 ml samples were in duplicate for Western and ChIP analysis.

References

  1. Tyler JK: Chromatin assembly. Cooperation between histone chaperones and ATP-dependent nucleosome remodeling machines. Eur J Biochem 2002, 269: 2268–2274. 10.1046/j.1432-1033.2002.02890.x

    Article  CAS  PubMed  Google Scholar 

  2. Verreault A: De novo nucleosome assembly: new pieces in an old puzzle. Genes Dev 2000, 14: 1430–8.

    CAS  PubMed  Google Scholar 

  3. Hagmann M: How chromatin changes its shape. Science 1999, 285: 1200–1203. 10.1126/science.285.5431.1200

    Article  CAS  PubMed  Google Scholar 

  4. Strahl BD, Allis CD: The language of covalent histone modifications. Nature 2000, 403: 41–45. 10.1038/47412

    Article  CAS  PubMed  Google Scholar 

  5. Brown CE, Lechner T, Howe L, Workman JL: The many HATs of transcription coactivators. Trends Biochem Sci 2000, 25: 15–19. 10.1016/S0968-0004(99)01516-9

    Article  CAS  PubMed  Google Scholar 

  6. Cheung WL, Briggs SD, Allis CD: Acetylation and chromosomal functions. Curr Opin Cell Biol 2000, 12: 326–333. 10.1016/S0955-0674(00)00096-X

    Article  CAS  PubMed  Google Scholar 

  7. Han Q, Lu J, Duan J, Su D, Hou X, Li F, Wang X, Huang B: Gcn5- and Elp3-induced histone H3 acetylation regulates hsp70 gene transcription in yeast. Biochem J 2008, 409: 779–788. 10.1042/BJ20070578

    Article  CAS  PubMed  Google Scholar 

  8. Sterner DE, Berger SL: Acetylation of histones and transcription-related factors. Microbiol Mol Biol Rev 2000, 64: 435–459. 10.1128/MMBR.64.2.435-459.2000

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. Struhl K: Histone acetylation and transcriptional regulatory mechanisms. Genes Dev 1998, 12: 599–606. 10.1101/gad.12.5.599

    Article  CAS  PubMed  Google Scholar 

  10. Kurdistani SM, Grunstein M: Histone acetylation and deacetylation in yeast. Nat Rev Mol Cell Biol 2003, 4: 276–284. 10.1038/nrm1075

    Article  CAS  PubMed  Google Scholar 

  11. Paranjape SM, Kamakaka RT, Kadonaga JT: Role of chromatin structure in the regulation of transcription by RNA polymerase II. Annu Rev Biochem 1994, 63: 265–297. 10.1146/annurev.bi.63.070194.001405

    Article  CAS  PubMed  Google Scholar 

  12. Krebs JE: Moving marks: dynamic histone modifications in yeast. Mol Biosyst 2007, 3: 590–597. 10.1039/b703923a

    Article  CAS  PubMed  Google Scholar 

  13. Khan SN, Khan AU: Role of histone acetylation in cell physiology and diseases: An update. Clin Chim Acta 2010, 411: 1401–1411. 10.1016/j.cca.2010.06.020

    Article  CAS  PubMed  Google Scholar 

  14. Vogelauer M, Wu J, Suka N, Grunstein M: Global histone acetylation and deacetylation in yeast. Nature 2000, 408: 495–498. 10.1038/35044127

    Article  CAS  PubMed  Google Scholar 

  15. Kan PY, Lu X, Hansen JC, Hayes JJ: The H3 tail domain participates in multiple interactions during folding and self-association of nucleosome arrays. Mol Cell Biol 2007, 27: 2084–2091. 10.1128/MCB.02181-06

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. Kan PY, Caterino TL, Hayes JJ: The H4 tail domain participates in intra- and internucleosome interactions with protein and DNA during folding and oligomerization of nucleosome arrays. Mol Cell Biol 2009, 29: 538–546. 10.1128/MCB.01343-08

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Shogren-Knaak M, Ishii H, Sun JM, Pazin MJ, Davie JR, Peterson CL: Histone H4-K16 acetylation controls chromatin structure and protein interactions. Science 2006, 311: 844–847. 10.1126/science.1124000

    Article  CAS  PubMed  Google Scholar 

  18. Lucchini G, Hinnebusch AG, Chen C, Fink GR: Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae . Mol Cell Biol 1984, 4: 1326–1333.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Brownell JE, Zhou J, Ranalli T, Kobayashi R, Edmondson DG, Roth SY, Allis CD: Tetrahymena histone acetyltransferase A: a transcriptional co-activator linking gene expression to histone acetylation. Cell 1996, 84: 843–851. 10.1016/S0092-8674(00)81063-6

    Article  CAS  PubMed  Google Scholar 

  20. Grant PA, Duggan L, Côté J, Roberts SM, Brownell JE, Candau R, Ohba R, Owen-Hughes T, Allis CD, Winston F, Berger SL, Workman JL: Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex. Genes Dev 1997, 11: 1640–1650. 10.1101/gad.11.13.1640

    Article  CAS  PubMed  Google Scholar 

  21. Baker SP, Grant PA: The SAGA continues: expanding the cellular role of a transcriptional co-activator complex. Oncogene 2007, 26: 5329–5340. 10.1038/sj.onc.1210603

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Pray-Grant MG, Schieltz D, McMahon SJ, Wood JM, Kennedy EL, Cook RG, Workman JL, Yates JR, Grant PA: The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway. Mol Cell Biol 2002, 22: 8774–8786. 10.1128/MCB.22.24.8774-8786.2002

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  23. Sendra R, Tse C, Hansen JC: The yeast histone acetyltransferase A2 complex, but not free Gcn5p, binds stably to nucleosomal arrays. J Biol Chem 2000, 275: 24928–24934. 10.1074/jbc.M003783200

    Article  CAS  PubMed  Google Scholar 

  24. Johnsson AE, Wright AP: The role of specific HAT-HDAC interactions in transcriptional elongation. Cell Cycle 2010, 9: 467–71. 10.4161/cc.9.3.10543

    Article  CAS  PubMed  Google Scholar 

  25. McCullough SD, Grant PA: Histone acetylation, acetyltransferases, and ataxia-alteration of histone acetylation and chromatin dynamics is implicated in the pathogenesis of polyglutamine-expansion disorders. Adv Protein Chem Struct Biol 2010, 79: 165–203.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. Koutelou E, Hirsch CL, Dent SY: Multiple faces of the SAGA complex. Curr Opin Cell Biol 2010, 22: 374–382. 10.1016/j.ceb.2010.03.005

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  27. Utley RT, Ikeda K, Grant PA, Cote J, Steger DJ, Eberharter A, John S, Workman JL: Transcriptional activators direct histone acetyltransferase complexes to nucleosomes. Nature 1998, 394: 498–502. 10.1038/28886

    Article  CAS  PubMed  Google Scholar 

  28. Rosaleny LE, Ruiz-Garcia AB, Garcia-Martinez J, Perez-Ortin JE, Tordera V: The Sas3p and Gcn5p histone acetyltransferases are recruited to similar genes. Genome Biol 2007, 8: R119. 10.1186/gb-2007-8-6-r119

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  29. Bhaumik SR, Raha T, Aiello DP, Green MR: In vivo target of a transcriptional activator revealed by fluorescence resonance energy transfer. Genes Dev 2004, 18: 333–343. 10.1101/gad.1148404

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  30. Wittschieben BO, Otero G, de Bizemont T, Fellows J, Erdjument-Bromage H, Ohba R, Li Y, Allis CD, Tempst P, Svejstrup JQ: A novel histone acetyltransferase is an integral subunit of elongating RNA polymerase II holoenzyme. Mol Cell 1999, 4: 123–128. 10.1016/S1097-2765(00)80194-X

    Article  CAS  PubMed  Google Scholar 

  31. Wittschieben BO, Fellows J, Du W, Stillman DJ, Svejstrup JQ: Overlapping roles for the histone acetyltransferase activities of SAGA and elongator in vivo . EMBO J 2000, 19: 3060–3068. 10.1093/emboj/19.12.3060

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  32. Krebs JE, Fry CJ, Samuels ML, Peterson CL: Global role for chromatin remodeling enzymes in mitotic gene expression. Cell 2000, 102: 587–598. 10.1016/S0092-8674(00)00081-7

    Article  CAS  PubMed  Google Scholar 

  33. Wirén M, Silverstein RA, Sinha I, Walfridsson J, Lee HM, Laurenson P, Pillus L, Robyr D, Grunstein M, Ekwall K: Genome wide analysis of nucleosome density histone acetylation and HDAC function in fission yeast. EMBO J 2005, 24: 2906–2918. 10.1038/sj.emboj.7600758

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  34. Dubief MD, Sinha I, Billai FF, Bonilla C, Wright A, Grunstein M, Ekwall K: Specific functions for the fission yeast Sirtuins Hst2 and Hst4 in gene regulation and retrotransposon silencing. EMBO J 2007, 26: 2477–2488. 10.1038/sj.emboj.7601690

    Article  CAS  Google Scholar 

  35. Kurdistani SK, Robyr D, Tavazoie S, Grunstein M: Genome-wide binding map of the histone deacetylase Rpd3 in yeast. Nat Genet 2002, 31: 248–254. 10.1038/ng907

    Article  CAS  PubMed  Google Scholar 

  36. Wu J, Suka N, Carlson M, Grunstein M: TUP1 utilizes histone H3/H2B-specific HDA1 deacetylase to repress gene activity in yeast. Mol Cell 2001, 7: 117–126. 10.1016/S1097-2765(01)00160-5

    Article  CAS  PubMed  Google Scholar 

  37. Robyr D, Suka Y, Xenarios I, Kurdistani SK, Wang A, Suka N, Grunstein M: Microarray deacetylation maps determine genome-wide functions for yeast histone deacetylases. Cell 2002, 109: 437–446. 10.1016/S0092-8674(02)00746-8

    Article  CAS  PubMed  Google Scholar 

  38. Komachi K, Redd M, Johnson A: The WD repeats of Tup1 interact with the homeo domain protein alpha 2. Genes Dev 1994, 8: 2857–2867. 10.1101/gad.8.23.2857

    Article  CAS  PubMed  Google Scholar 

  39. Tzamarias D, Struhl K: Distinct TPR motifs of Cyc8 are involved in recruiting the Cyc8-Tup1 corepressor complex to differentially regulated promoters. Genes Dev 1995, 9: 821–831. 10.1101/gad.9.7.821

    Article  CAS  PubMed  Google Scholar 

  40. Kobayashi Y, Inai T, Mizunuma M, Okada I, Shitamukai A, Hirata D, Miyakawa T: Identification of Tup1 and Cyc8 mutations defective in the responses to osmotic stress. Biochem Biophys Res Commun 2008, 368: 50–55. 10.1016/j.bbrc.2008.01.033

    Article  CAS  PubMed  Google Scholar 

  41. Zhang Z, Reese JC: Redundant Mechanisms Are Used by Ssn6-Tup1 in Repressing Chromosomal Gene Transcription in Saccharomyces cerevisiae . J Biol Chem 2004, 279: 39240–39250. 10.1074/jbc.M407159200

    Article  CAS  PubMed  Google Scholar 

  42. Bone JR, Roth SY: Recruitment of the yeast Tup1p-Ssn6p repressor is associated with localized decreases in histone acetylation. J Biol Chem 2001, 276: 1808–1813.

    Article  CAS  PubMed  Google Scholar 

  43. Deckert J, Struhl K: Histone acetylation at promoters is differentially affected by specific activators and repressors. Mol Cell Biol 2001, 21: 2726–2735. 10.1128/MCB.21.8.2726-2735.2001

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  44. Davie JK, Trumbly RJ, Dent SY: Histone-dependent association of Tup1-Ssn6 with repressed genes in vivo . Mol Cell Biol 2002, 22: 693–703. 10.1128/MCB.22.3.693-703.2002

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  45. Watson AD, Edmondson DG, Bone JR, Mukai Y, Yu Y, Du W, Stillman DJ, Roth SY: Ssn6-Tup1 interacts with class I histone deacetylases required for repression. Genes Dev 2000, 14: 2737–2744. 10.1101/gad.829100

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  46. Davie JK, Edmondson DG, Coco CB, Dent SY: Tup1-Ssn6 interacts with multiple class I histone deacetylases in vivo . J Biol Chem 2003, 278: 50158–50162. 10.1074/jbc.M309753200

    Article  CAS  PubMed  Google Scholar 

  47. Smith RL, Johnson AD: Turning genes off by Ssn6-Tup1: a conserved system of transcriptional repression in eukaryotes. Trends Biochem Sci 2000, 25: 325–330. 10.1016/S0968-0004(00)01592-9

    Article  CAS  PubMed  Google Scholar 

  48. Malavé TM, Dent SY: Transcriptional repression by Tup1-Ssn6. Biochem. Cell Biol 2006, 84: 437–443.

    Google Scholar 

  49. Harper JW, Burton JL, Solomon MJ: The anaphase-promoting complex: it's not just for mitosis any more. Genes Dev 2002, 16: 2179–2206. 10.1101/gad.1013102

    Article  CAS  PubMed  Google Scholar 

  50. Manchado E, Eguren M, Malumbres M: The anaphase-promoting complex/cyclosome (APC/C): cell-cycle-dependent and -independent functions. Biochem Soc Trans 2010, 38: 65–71. 10.1042/BST0380065

    Article  CAS  PubMed  Google Scholar 

  51. Buchakjian MR, Kornbluth S: The engine driving the ship: metabolic steering of cell proliferation and death. Nat Rev Mol Cell Biol 2010, 11: 715–27. 10.1038/nrm2972

    Article  CAS  PubMed  Google Scholar 

  52. Qiao X, Zhang L, Gamper AM, Fujita T, Wan Y: APC/C-Cdh1: from cell cycle to cellular differentiation and genomic integrity. Cell Cycle 2010, 9: 3904–12. 10.4161/cc.9.19.13585

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  53. Arnason TG, Pisclevich MG, Dash MD, Davies GF, Harkness TA: Novel interaction between Apc5p and Rsp5p in an intracellular signaling pathway in Saccharomyces cerevisiae . Eukaryot Cell 2005, 4: 134–146. 10.1128/EC.4.1.134-146.2005

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  54. Harkness TA, Davies GF, Ramaswamy V, Arnason TG: The ubiquitin-dependent targeting pathway in Saccharomyces cerevisiae plays a critical role in multiple chromatin assembly regulatory steps. Genetics 2002, 162: 615–632.

    PubMed Central  CAS  PubMed  Google Scholar 

  55. Harkness TA, Arnason TG, Legrand C, Pisclevich MG, Davies GF, Turner EL: Contribution of CAF-I to anaphase-promoting-complex-mediated mitotic chromatin assembly in Saccharomyces cerevisiae . Eukaryot Cell 2005, 4: 673–684. 10.1128/EC.4.4.673-684.2005

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  56. Harkness TA: Chromatin assembly from yeast to man: Conserved factors and conserved molecular mechanisms. Curr Genomics 2005, 6: 227–240. 10.2174/1389202054395937

    Article  CAS  Google Scholar 

  57. Turner EL, Malo ME, Pisclevich MG, Dash MD, Harkness TA: The Anaphase Promoting Complex interacts with multiple histone modifying enzymes to regulate cell cycle progression in yeast. Eukaryot Cell 2010, 9: 1418–1431. 10.1128/EC.00097-10

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  58. Kotani S, Tanaka H, Yasuda H, Todokoro K: Regulation of APC Activity by Phosphorylation and Regulatory Factors. J Cell Biol 1998, 146: 791–800.

    Article  Google Scholar 

  59. Rudner AD, Murray AW: Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex. J Cell Biol 2000, 149: 1377–1390. 10.1083/jcb.149.7.1377

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  60. Harkness TA, Shea KA, Legrand C, Brahmania M, Davies GF: A functional analysis reveals dependence on the anaphase-promoting complex for prolonged life span in yeast. Genetics 2004, 168: 759–774. 10.1534/genetics.104.027771

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  61. Nasmyth K: How do so few control so many? Cell 2005, 120: 739–746. 10.1016/j.cell.2005.03.006

    Article  CAS  PubMed  Google Scholar 

  62. Suijkerbuijk SJ, Kops GJ: Preventing aneuploidy: the contribution of mitotic checkpoint proteins. Biochim Biophys Acta 2008, 1786: 24–31.

    CAS  PubMed  Google Scholar 

  63. Simonetta M, Manzoni R, Mosca R, Mapelli M, Massimiliano L, Vink M, Novak B, Musacchio A, Ciliberto A: The influence of catalysis on mad2 activation dynamics. PLoS Biol 2009, 7: e10. 10.1371/journal.pbio.1000010

    Article  PubMed  CAS  Google Scholar 

  64. Benhamed M, Bertrand C, Servet C, Zhou DX: Arabidopsis GCN5, HD1, and TAF1/HAF2 interact to regulate histone acetylation required for light-responsive gene expression. Plant Cell 2006, 18: 2893–2903. 10.1105/tpc.106.043489

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  65. Vetting MW, Magnet S, Nieves E, Roderick SL, Blanchard JS: A bacterial acetyltransferase capable of regioselective N-acetylation of antibiotics and histones. Chem Biol 2004, 11: 565–573. 10.1016/j.chembiol.2004.03.017

    Article  CAS  PubMed  Google Scholar 

  66. Landry J, Sutton A, Tafrov ST, Heller RC, Stebbins J, Pillus L, Sternglanz R: The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases. Proc Natl Acad Sci USA 2000, 97: 5807–5811. 10.1073/pnas.110148297

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  67. Vogelauer M, Wu J, Suka N, Grunstein M: Global histone acetylation and deacetylation in yeast. Nature 2000, 408: 495–498. 10.1038/35044127

    Article  CAS  PubMed  Google Scholar 

  68. Green SR, Johnson AD: Promoter-dependent roles for the Srb10 cyclin-dependent kinase and the Hda1 deacetylase in Tup1-mediated repression in Saccharomyces cerevisiae . Mol Biol Cell 2004, 15: 4191–4202. 10.1091/mbc.E04-05-0412

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  69. Lee TI, Causton HC, Holstege FC, Shen WC, Hannett N, Jennings EG, Winston F, Green MR, Young RA: Redundant roles for the TFIID and SAGA complexes in global transcription. Nature 2000, 405: 701–704. 10.1038/35015104

    Article  CAS  PubMed  Google Scholar 

  70. Koç A, Wheeler LJ, Mathews CK, Merrill GF: Replication-independent MCB gene induction and deoxyribonucleotide accumulation at G1/S in Saccharomyces cerevisiae . J Biol Chem 2003, 278: 9345–9352. 10.1074/jbc.M213013200

    Article  PubMed  CAS  Google Scholar 

  71. Imoberdorf RM, Topalidou I, Strubin M: A role for gcn5-mediated global histone acetylation in transcriptional regulation. Mol Cell Biol 2006, 26: 1610–1616. 10.1128/MCB.26.5.1610-1616.2006

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  72. Larschan E, Winston F: The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription. Mol Cell Biol 2005, 25: 114–123. 10.1128/MCB.25.1.114-123.2005

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  73. Papamichos-Chronakis M, Petrakis T, Ktistaki E, Topalidou I, Tzamarias D: Cti6, a PHD domain protein, bridges the Cyc8-Tup1 corepressor and the SAGA coactivator to overcome repression at GAL1. Mol Cell 2002, 9: 1297–1305. 10.1016/S1097-2765(02)00545-2

    Article  CAS  PubMed  Google Scholar 

  74. Proft M, Struhl K: Hog1 kinase converts the Sko1-Cyc8-Tup1 repressor complex into an activator that recruits SAGA and SWI/SNF in response to osmotic stress. Mol Cell 2002, 9: 1307–1317. 10.1016/S1097-2765(02)00557-9

    Article  CAS  PubMed  Google Scholar 

  75. DeSimone AM, Laney JD: Corepressor-directed preacetylation of histone H3 in promoter chromatin primes rapid transcriptional switching of cell-type-specific genes in yeast. Mol Cell Biol 2010, 30: 3342–3356. 10.1128/MCB.01450-09

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  76. Lin YY, Qi Y, Lu JY, Pan X, Yuan DS, Zhao Y, Bader JS, Boeke JD: A comprehensive synthetic genetic interaction network governing yeast histone acetylation and deacetylation. Genes Dev 2008, 22: 2062–2074. 10.1101/gad.1679508

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  77. Costanzo M, Baryshnikova A, Bellay J, Kim Y, Spear ED, Sevier CS, Ding H, Koh JL, Toufighi K, Mostafavi S, Prinz J, St Onge RP, VanderSluis B, Makhnevych T, Vizeacoumar FJ, Alizadeh S, Bahr S, Brost RL, Chen Y, Cokol M, Deshpande R, Li Z, Lin ZY, Liang W, Marback M, Paw J, San Luis BJ, Shuteriqi E, Tong AH, van Dyk N, Wallace IM, Whitney JA, Weirauch MT, Zhong G, Zhu H, Houry WA, Brudno M, Ragibizadeh S, Papp B, Pál C, Roth FP, Giaever G, Nislow C, Troyanskaya OG, Bussey H, Bader GD, Gingras AC, Morris QD, Kim PM, Kaiser CA, Myers CL, Andrews BJ, Boone C: The genetic landscape of a cell. Science 2010, 327: 425–431. 10.1126/science.1180823

    Article  CAS  PubMed  Google Scholar 

  78. Edmondson DG, Smith MM, Roth SY: Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4. Genes Dev 1996, 10: 1247–1259. 10.1101/gad.10.10.1247

    Article  CAS  PubMed  Google Scholar 

  79. Fillingham J, Recht J, Silva AC, Suter B, Emili A, Stagljar I, Krogan NJ, Allis CD, Keogh MC, Greenblatt JF: Chaperone control of the activity and specificity of the histone H3 acetyltransferase Rtt109. Mol Cell Biol 2008, 28: 4342–4353. 10.1128/MCB.00182-08

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  80. Vernarecci S, Ornaghi P, Bâgu A, Cundari E, Ballario P, Filetici P: Gcn5p plays an important role in centromere kinetochore function in budding yeast. Mol Cell Biol 2008, 28: 988–996. 10.1128/MCB.01366-07

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  81. Kanta H, Laprade L, Almutairi A, Pinto I: Suppressor analysis of a histone defect identifies a new function for the hda1 complex in chromosome segregation. Genetics 2006, 173: 435–450. 10.1534/genetics.105.050559

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  82. Rundlett SE, Carmen AA, Suka N, Turner BM, Grunstein M: Transcriptional repression by UME6 involves decaetylation of lysine 5 of histone H4 by RPD3. Nature 1998, 392: 831–835. 10.1038/33952

    Article  CAS  PubMed  Google Scholar 

  83. Suka N, Nakashima E, Shinmyozu K, Hidaka M, Jingami H: The WD40-repeat protein Pwp1p associates with 25S ribosomal chromatin in a histone H4 tail-dependent manner. Nucleic Acid Res 2006, 34: 3555–3567. 10.1093/nar/gkl487

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  84. Schnekenburger M, Peng L, Puga A: HDAC1 bound to the Cyp1a1 promoter blocks histone acetylation associated with Ah receptor-mediated trans-activation. Biochim Biophys Acta 2007, 1769: 569–578.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Acknowledgements

AI was supported by Post-Doctoral Fellowships from the Saskatchewan Health Research Foundation (SHRF), and from the Canadian Institutes for Health Research-Regional Partnership Program (CIHR-RPP). ELT was supported by Graduate Scholarships from the College of Graduate Studies at the U of S. Deletion mutants and plasmids were kindly provided by Dr. W. Xiao (University of Saskatchewan). We thank members of the Harkness lab, Ata Ghavidel and Spike Postnikoff, for careful reading of the manuscript and for providing insightful suggestions. Funding was generously provided to TAAH through a CIHR Operating Grant and a New Investigator Award from the Canadian Foundation for Innovation (CFI).

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Correspondence to Troy AA Harkness.

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AI conducted the vast majority of the experiments described in this manuscript. ELT discovered the interaction between apc5CA , gcn5 Δ and hda1 Δ while performing the genetic screen designed to identify HAT and HDAC mutants that genetically interact with apc5CA [57]. ELT, MEM and JM contributed to work demonstrating accumulation of Gcn5 and Elp3 in ubiquitin compromised strains. TAAH designed the study, supervised the work, and wrote the manuscript. All authors approve the final manuscript.

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Islam, A., Turner, E.L., Menzel, J. et al. Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast. Cell Div 6, 13 (2011). https://doi.org/10.1186/1747-1028-6-13

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