Methodology
Live cell division dynamics monitoring in 3D large spheroid tumor models using light sheet microscopy
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* Corresponding authors: Bernard Ducommun bernard.ducommun@itav-recherche.fr - Valérie Lobjois valerie.lobjois@itav-recherche.fr
1 Université de Toulouse; ITAV-UMS3039, F-31106 Toulouse, France
2 CNRS; ITAV-UMS3039, F-31106 Toulouse, France
3 Université de Toulouse; IMT-UMR5219, F-31062 Toulouse, France
4 CNRS; IMT-UMR5219, F-31062 Toulouse, France
5 CHU de Toulouse; F-31059 Toulouse, France
Cell Division 2011, 6:22 doi:10.1186/1747-1028-6-22
Published: 12 December 2011Abstract
Background
Multicellular tumor spheroids are models of increasing interest for cancer and cell biology studies. They allow considering cellular interactions in exploring cell cycle and cell division mechanisms. However, 3D imaging of cell division in living spheroids is technically challenging and has never been reported.
Results
Here, we report a major breakthrough based on the engineering of multicellular tumor spheroids expressing an histone H2B fluorescent nuclear reporter protein, and specifically designed sample holders to monitor live cell division dynamics in 3D large spheroids using an home-made selective-plane illumination microscope.
Conclusions
As illustrated using the antimitotic drug, paclitaxel, this technological advance paves the way for studies of the dynamics of cell divion processes in 3D and more generally for the investigation of tumor cell population biology in integrated system as the spheroid model.