Additional file 1.

The Selective Plane Illumination Microscopy (SPIM) setup. A: Schematics of the SPIM system. Lateral (B) and top (C) views of the SPIM setup. The green path corresponds to the illumination axis and the perpendicular red path corresponds to the detection axis. The sample is suspended through the theta-stage (θS) in the physiological chamber (PC) and is positioned in the focal plane of the detection objective. It can be moved in the x, y and z axis and rotated (θ axis, blue arrow in c). T: telescope; M: mirror; CL: cylindrical lens; TL: tube lens; IO: illumination objective; PC: physiological chamber; XS: X-stage; YS: Y-stage; ZS: Z-stage, θS: θstage; F and FW: filter wheel. D: The physiological chamber was designed using the Open source Blender software (top) and then manufactured by stereolithography of a photosensitive epoxy resin (bottom). The two tinny holes on the top of the chamber (red circles) allow to inject CO2 directly in the physiological chamber at the surface of the culture medium.

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Lorenzo et al. Cell Division 2011 6:22   doi:10.1186/1747-1028-6-22